Animals (specifically a dog) are seen in peril. May be difficult or upsetting for animal lovers.
Overall, the show plays most scenes in a comedic way, but towards the end of the show it starts to get more serious.
A disused fridge is shown from the inside with slime, fungus, mold, bacteria and decaying matter. It's all shown in great detail and in close-up, and it can be disgusting for some.
Scenes of action violence are presented in a light, rather comedic way but other scenes involving violence have a emotional feel.
Some flashbacks of the crew can be quite saddening.
Although most of the anime is fairly lighthearted and comedic, it occasionally deals with a bunch of profound and complex themes such as existentialism, existential boredom, loneliness, environmentalism and capitalism, and it also features troubled characters with emotional backstories that explore how they became the way they are.
Episode 20 of the show is a horror episode. So, it's naturally terrifying.
Spike is seen with lots of blood on his face in the final episode.
The backstories of certain characters can be emotional. As their family members have mostly passed away which could be upsetting.