Wilbur jumps off a hay bale (trying to "spin a web" like Charlotte) and hits the ground, he jumps the same hay bale again but with his tail tied to ceiling wood with a piece of string. It then snaps and he then falls and hits the ground again. (Nothing graphic, but made for laughs)
Templeton, the rat, bites Wilbur's tail on a couple of occasions in order to revive him from having fainted, after one or more other animals encourage him to revive Wilbur.
Wilbur is almost beheaded in the beginning of the movie.
There is talk about death and being killed for bacon and ham in the movie.
Charlotte sucks blood out of insects in her web.